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ACCESSION NO: 1006707 [Full Record]
PROJ NO: SC-2014-10231 AGENCY: NIFA SC.
PROJ TYPE: AFRI COMPETITIVE GRANT PROJ STATUS: TERMINATED
CONTRACT/GRANT/AGREEMENT NO: 2015-67031-23795 PROPOSAL NO: 2014-10231
START: 01 AUG 2015 TERM: 31 JUL 2020 FY: 2019
GRANT AMT: $99,987 GRANT YR: 2015
AWARD TOTAL: $99,987
INITIAL AWARD YEAR: 2015
INVESTIGATOR: Greene, A.
PERFORMING INSTITUTION:
CLEMSON UNIVERSITY
CLEMSON, SOUTH CAROLINA 29634
UNINTENTIONAL CREATION OF A HEAT RESISTANT BACTERIAL BIOTA AND ITS IMPACT ON FOOD SAFETY
NON-TECHNICAL SUMMARY: Rendering processes waste animal tissues into animal feed ingredients that are recycled to poultry, swine, cattle and pets. With more than 8 billion chickens, 100 million hogs and 35 million cattle currently slaughtered annually, the rendering industry provides a significant benefit to society by processing billions of pounds of perishable waste tissues into stable value-added products while protecting the environment. The majority of rendered products are utilized as animal feed ingredients. In previous studies in this laboratory, a very low population of a heat resistant biota has been isolated from rendered animal products. The question is if recycling of materials has been a selective process creating a mobile biota of thermophilic microorganisms. It is important to learn more about this biota for cautionary monitoring and/or predictive
actions of this biota to ensure human and animal health and safety. This proposed study will confirm the identify of the adapted thermophiles through genome sequencing, determine if these organisms could be pathogenic to animals and/or humans, and determine if horizontal gene transfer of virulence factors, such as antibiotic resistance, has occurred.
OBJECTIVES: Major Goals: Measure and assess the structure and complexity of the biodiversity present in rendered animal products that are used for animal feed and pet food ingredients.Objective 1: Sequence the genomes of resistant strains of Salmonella isolated from rendered animal products to define and interpret the level of dynamic gene-transfer between living heat resistant populations of bacteria after rendering and known reference pathogenic strains of bacteria.Objective 2: Identify and characterize the functional elements of post rendering biota, which may encode enzymes and other bacterial by-products with industrial uses.
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