|
Item No. 1 of 1
ACCESSION NO: 1008811 [Full Record]
PROJ NO: MISV-371720 AGENCY: NIFA MISV
PROJ TYPE: AFRI COMPETITIVE GRANT PROJ STATUS: TERMINATED
CONTRACT/GRANT/AGREEMENT NO: 2016-67015-24909 PROPOSAL NO: 2015-07054
START: 01 FEB 2016 TERM: 31 JAN 2020 FY: 2019
GRANT AMT: $460,000 GRANT YR: 2016
AWARD TOTAL: $460,000
INITIAL AWARD YEAR: 2016
INVESTIGATOR: Karsi, A.
PERFORMING INSTITUTION:
MISSISSIPPI STATE UNIV
MISSISSIPPI STATE, MISSISSIPPI 39762
PREVENTION OF ENTERIC SEPTICEMIA OF CATFISH BY NOVEL LIVE ATTENUATED VACCINES
NON-TECHNICAL SUMMARY: Channel catfish farming is the largest aquaculture industry in the U.S., and Edwardsiella ictaluri is recognized as the most economically important bacterial pathogen of catfish. The type six secretion system (T6SS) and universal stress proteins (USP) are essential for E. ictaluri virulence in catfish. However, critical work needs to be conducted to develop safe and efficacious live attenuated vaccines (LAV) against E. ictaluri. Our long-term goal is to characterize mechanisms of E. ictaluri pathogenesis in order to develop effective control strategies for the disease. The immediate objective of this study is to develop novel LAVs by mutating T6SS and USP genes and by characterizing mutants' safety and efficacy in catfish. The rationale of the proposed research is that T6SS and USPs are essential for E. ictaluri virulence in catfish and
mutation of genes in these systems is expected to yield safe and efficacious vaccines for catfish. This study is significant because of the large economic impact enteric septicemia has on the catfish industry. Current disease treatments are often ineffective, costly, and can result in drug resistance or environmental contamination. Therefore, vaccine-based strategies for control of diseases are essential to support the development of an environmentally and economically sustainable aquaculture industry in the U.S.
OBJECTIVES: Construct T6SS and USP mutantsCharacterize T6SS and USP mutants
|